forward genetics

Forward and Reverse Genetic Tools for Investigating Gene Function

Genetics and molecular genomic approaches have been changed our perception towards biological processes. Reverse genetics describes the “gene to phenotype”approach, with which functions of a gene of interest can be examined by disrupting the gene expression at physiological level. Forward genetics is a “phenotype to gene” approach with which genes involved in a biological pathway can be identified through the screening of populations that contain random modifications throughout the genome, altering the gene function.