Agricultural sector is play a vital role in the Sudan economy and is considered to be the backbone of industrial development in the country. Sudan lost both south Sudan and oil revenues, the poverty increased among Sudanese. Therefore, the aim of this paper is to examine the role of agriculture in post-separation development in Sudan. However, it missed opportunities on a number of occasions due to inappropriate economic policies, continues recent region conflicts. The key massage from the studies examined in this paper is that agricultural sector in Sudan still face many challenges.