IN-SILICO PROTEIN STRUCTURE PREDICTION:HOMOLOGY MODELLING-A REVIEW
Proteins are chains of amino acid joined by hydrogen bond. The linear chain forms secondary structures through hydrogen bond between amino acid in the linear chain. Through further interactions among amino acid groups the secondary structures fold to form three dimensional structures. Thus the structure of protein is largely determined by amino acid sequence. So efforts are made to predict the structure of protein.
One of the major goals of Bioinformatics is to understand the relationship between amino acid sequence and three-dimensional structure of proteins. If this relationship were understood then the three-dimensional structure of protein could be easily predicted from the amino acid sequence which reveals the function of the protein. Unfortunately understanding this relation is not an easy task and thus progress has been made to categorize proteins on the basis of their structure and amino acid sequence. This facilitates in protein modeling. Thus this experimental work is carried out by the Author to predict the three-dimensional structure of a mutant protein using Bioinformatics approach which is economic, time saving and cost-effective. The predicted structure has been evaluated using various tools and softwares to check the accuracy of the predicted structure. The predicted structure is then compared with the wild-type protein structure which has been resolved experimentally and significant conclusions are drawn thereof.